Deposition of samples and sample matrix for enhancing the sensitivity of matrix-assisted laser desorption/ionization mass spectrometry

The present invention relates to small sample spots for MALDI targets and methods for producing the same. The sample spots are composed of a layer of matrix material and a layer of analyte. In some instances, the sample spots have diameters of no more than 50 micrometers or even smaller. The sample spots are deposited onto a MALDI target substrate using ultrasonic deposition from a nozzle.